ELISA Kit for Brain Natriuretic Peptide (BNP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

24.69-2,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 9.67pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Brain Natriuretic Peptide (BNP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Brain Natriuretic Peptide (BNP) and unlabeled Brain Natriuretic Peptide (BNP) (Standards or samples) with the pre-coated antibody specific to Brain Natriuretic Peptide (BNP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Brain Natriuretic Peptide (BNP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Brain Natriuretic Peptide (BNP) in the sample.

Alternative Names

GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32

Item Name

Brain Natriuretic Peptide

Research Area

Cardiovascular biology;Neuro science;Hormone metabolism;

Reference

B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sickness;ELEVATED ST2 SERUM LEVELS A BIOMARKER FOR CARDIOMYOPATHY IN DUCHENNE MUSCULAR DYSTROPHY;Why does Adenotonsillectomy Not Correct Enuresis in All Children with Sleep Disordered Breathing?;Improvement in the prediction of exercise-induced elevation of left ventricular filling pressure in patients with normal left ventricular ejection fraction.;Plasma concentration of fibronectin is decreased in patients with hypertrophic cardiomyopathy;High-intensity interval training enhances mitochondrial bioenergetics of platelets in patients with heart failure;Determinants of Increased Aortic Diameters in Young Normotensive Patients With Turner Syndrome Without Structural Heart Disease;Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure;Inflammatory cytokines associated with cancer growth induce mitochondria and cytoskeleton alterations in cardiomyocytes;