ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

31.2-2,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 11.3pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Derived Neurotrophic Factor (BDNF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Brain Derived Neurotrophic Factor (BDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brain Derived Neurotrophic Factor (BDNF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Brain Derived Neurotrophic Factor (BDNF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

Neurotrophin; Abrineurin

Item Name

Brain Derived Neurotrophic Factor

Research Area

Cytokine;Infection immunity;Neuro science;

Reference

Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells;Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities;Long‐term lithium treatment increases intracellular and extracellular brain‐derived neurotrophic factor (BDNF) in cortical and hippocampal neurons at subtherapeutic …;A new comorbidity model and the common pathological mechanisms of migraine and epilepsy;Changes in exercise capacity and serum BDNF following long-term sprint interval training in well-trained cyclists;Establishment and evaluation of a novel mouse model of peri/postmenopausal depression;Physical Exercise Increased Brain-Derived Neurotrophic Factor in Elderly Population with Depression;