ELISA Kit for Beta-2-Microglobulin (b2M)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.41-300ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.16ng/mL

Sample type

Serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-2-Microglobulin (b2M). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta-2-Microglobulin (b2M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-2-Microglobulin (b2M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-2-Microglobulin (b2M) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

BMG; B2MG

Item Name

Beta-2-Microglobulin

Research Area

Infection immunity;Kidney biomarker;

Reference

Absence of chloride intracellular channel 4 (CLIC4) predisposes to acute kidney injury but has minimal impact on recovery;Mef2C restrains microglial inflammatory response and is lost in brain ageing in an IFN-I-dependent manner.;