ELISA Kit for Atrial Natriuretic Peptide (ANP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 5.11pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Atrial Natriuretic Peptide (ANP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Atrial Natriuretic Peptide (ANP) and unlabeled Atrial Natriuretic Peptide (ANP) (Standards or samples) with the pre-coated antibody specific to Atrial Natriuretic Peptide (ANP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Atrial Natriuretic Peptide (ANP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Atrial Natriuretic Peptide (ANP) in the sample.

Alternative Names

ANH; ANF; CDD; Atrial Natriuretic Factor; Atrial Natriuretic Hormone; Atriopeptin; Cardionatrine; Cardiodilatin

Item Name

Atrial Natriuretic Peptide

Research Area

Endocrinology;Cardiovascular biology;Hormone metabolism;

Reference

Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity and;Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure;Relationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure.;