ELISA Kit for Apolipoprotein E (APOE)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

15.6-1,000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Oryctolagus cuniculus (Rabbit)

Sensitivity

The minimum detectable dose of this kit is typically less than 6.7ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein E (APOE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein E (APOE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein E (APOE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein E (APOE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset

Item Name

Apolipoprotein E

Research Area

Metabolic pathway;Cardiovascular biology;Hepatology;

Reference