ELISA Kit for Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

3.12-200ng/mL

Application

Enzyme-linked immunosorbent assay for Antibody Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 1.13ng/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h, 30min

Method

Competitive Inhibition

Test principle

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2

Item Name

Tumor Necrosis Factor Alpha

Research Area

Cytokine;Tumor immunity;Infection immunity;

Reference