ELISA Kit for Anti-Rh Blood Group, D Antigen Antibody (Anti-RHD)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

3.12-200ng/mL

Application

Enzyme-linked immunosorbent assay for Antibody Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 1.27ng/mL

Sample type

serum, plasma and other biological fluids

Assay length

2h, 30min

Method

Competitive Inhibition

Test principle

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Rh Blood Group, D Antigen Antibody (Anti-RHD) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Rh Blood Group, D Antigen Antibody (Anti-RHD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CD240D; Rh30a; Rh4; RhPI; RhII; DIIIc; Rhesus D antigen; Rh polypeptide 2

Item Name

Rh Blood Group, D Antigen

Research Area

Hematology;

Reference