ELISA Kit for Anti-Mullerian Hormone (AMH)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

61.73-5,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Bos taurus; Bovine (Cattle)

Sensitivity

The minimum detectable dose of this kit is typically less than 24.77pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Anti-Mullerian Hormone (AMH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Anti-Mullerian Hormone (AMH) and unlabeled Anti-Mullerian Hormone (AMH) (Standards or samples) with the pre-coated antibody specific to Anti-Mullerian Hormone (AMH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Anti-Mullerian Hormone (AMH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Anti-Mullerian Hormone (AMH) in the sample.

Alternative Names

MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance

Item Name

Anti-Mullerian Hormone

Research Area

Endocrinology;Reproductive science;Hormone metabolism;

Reference

Age-related changes in transcriptional abundance and circulating levels of anti-Mullerian hormone and Sertoli cell count in crossbred and Zebu bovine males.;Relationship among circulating anti-Müllerian hormone, insulin like growth factor 1, cadmium and superovulatory response in dairy cows.;