ELISA Kit for Angiotensin II Receptor Associated Protein (AGTRAP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

78-5,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 31pg/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin II Receptor Associated Protein (AGTRAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiotensin II Receptor Associated Protein (AGTRAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin II Receptor Associated Protein (AGTRAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiotensin II Receptor Associated Protein (AGTRAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

ATRAP; Type-1 angiotensin II receptor-associated protein; AT1 receptor-associated protein

Item Name

Angiotensin II Receptor Associated Protein

Research Area

Signal transduction;Metabolic pathway;Endocrinology;Cardiovascular biology;Hormone metabolism;

Reference