ELISA Kit for Angiotensin 1-7 (Ang1-7)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

9.88-800pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.34pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Angiotensin 1-7 (Ang1-7) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Angiotensin 1-7 (Ang1-7) and unlabeled Angiotensin 1-7 (Ang1-7) (Standards or samples) with the pre-coated antibody specific to Angiotensin 1-7 (Ang1-7). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Angiotensin 1-7 (Ang1-7) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Angiotensin 1-7 (Ang1-7) in the sample.

Alternative Names

Item Name

Angiotensin 1-7

Research Area

Reference

Improvement of Plasma Biomarkers after Switching Stroke Patients from Other Angiotensin II Type I Receptor Blockers to Olmesartan;Mesenchymal stem cell–derived microvesicles alleviate pulmonary arterial hypertension by regulating renin-angiotensin system;Lipoxin A attenuates LPS-induced acute lung injury via activation of the ACE2-Ang-(1-7)-Mas axis;Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects;