ELISA Kit for Angiotensin 1-7 (Ang1-7)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

2.47-200pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.91pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluid

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Angiotensin 1-7 (Ang1-7) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Angiotensin 1-7 (Ang1-7) and unlabeled Angiotensin 1-7 (Ang1-7) (Standards or samples) with the pre-coated antibody specific to Angiotensin 1-7 (Ang1-7). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Angiotensin 1-7 (Ang1-7) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Angiotensin 1-7 (Ang1-7) in the sample.

Alternative Names

Item Name

Angiotensin 1-7

Research Area

Reference

Angiotensin-(1-7) in Paraventricular Nucleus Contributes to the Enhanced Cardiac Sympathetic Afferent Reflex and Sympathetic Activity in Chronic Heart Failure Rats;The ACE2-Ang (1-7)-Mas receptor axis attenuates cardiac remodeling and fibrosis in post-myocardial infarction.;Effects of IQP, VEP and Spirulina platensis hydrolysates on the local kidney renin angiotensin system in spontaneously hypertensive rats;A Study of the Correlation between Angiotensin (1-7) and the Histopathological Changes in the Penises of Experimentally Diabetic Rats;