ELISA Kit for Angiopoietin Like Protein 8 (ANGPTL8)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.058ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin Like Protein 8 (ANGPTL8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiopoietin Like Protein 8 (ANGPTL8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiopoietin Like Protein 8 (ANGPTL8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiopoietin Like Protein 8 (ANGPTL8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

RIFL; TD26; C19orf80; Lipasin; Betatrophin; Hepatocellular Carcinoma-Associated Protein TD26

Item Name

Angiopoietin Like Protein 8

Research Area

Developmental science;

Reference

Increased Maternal and Cord Blood Betatrophin in Gestational Diabetes;Serum betatrophin level increased in subjects with metabolic syndrome: a case-control study;Serum betatrophin level increased in subjects with nonalcoholic fatty liver disease;Relationship between serum betatrophin levels and the first-phase of glucose-stimulated insulin secretion;Aumento del nivel de betatrofina en suero en sujetos con síndrome metabólico: estudio de casos y controles;Levels of betatrophin decrease during pregnancy despite increased insulin resistance, beta-cellfunction and triglyceride levels.;Decreased circulating levels of betatrophin in Chinese women with polycystic ovary syndrome;Serum concentrations of betatrophin and its association with indirect indices of insulin resistance and beta cell function in women with polycystic ovary …;Angiopoietin-like protein 8: an attractive biomarker for the evaluation of subjects with insulin resistance and related disorders;ANGPTL8 (Betatrophin) Role in Diabetes and Metabolic Diseases;The Correlation Between Circulating Betatrophin and Insulin Resistance in General Population: A Meta-Analysis;ANGPTL8: an important regulator in metabolic disorders;