ELISA Kit for Androstenediol (AED)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

123.5-10000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 53.4pg/mL

Sample type

serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Androstenediol (AED) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Androstenediol (AED) and unlabeled Androstenediol (AED) (Standards or samples) with the pre-coated antibody specific to Androstenediol (AED). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Androstenediol (AED) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Androstenediol (AED) in the sample.

Alternative Names

5-AED; 5AED; 5-Androstenediol; Androst-5-Ene-3Beta,17Beta-Diol

Item Name

Androstenediol

Research Area

Endocrinology;Reproductive science;Hormone metabolism;

Reference

The role of androst-5-ene-3β,17β-diol (androstenediol) in cell proliferation in endometrium of women with polycystic ovary syndrome;