ELISA Kit for Amyloid Beta Peptide 1-42 (Ab1-42)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

6.17-500pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 2.55pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Amyloid Beta Peptide 1-42 (Ab1-42) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Amyloid Beta Peptide 1-42 (Ab1-42) and unlabeled Amyloid Beta Peptide 1-42 (Ab1-42) (Standards or samples) with the pre-coated antibody specific to Amyloid Beta Peptide 1-42 (Ab1-42). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Amyloid Beta Peptide 1-42 (Ab1-42) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Amyloid Beta Peptide 1-42 (Ab1-42) in the sample.

Alternative Names

Item Name

Amyloid Beta Peptide 1-42

Research Area

Neuro science;

Reference

Blockade of Tau Hyperphosphorylation and Aβ1–42 Generation by the Aminotetrahydrofuran Derivative ANAVEX2-73, a Mixed Muscarinic and σ1 Receptor Agonist, in a Nontransgenic Mouse Model of Alzheimer’s Disease;The γ-secretase inhibitor 2-[(1R)-1-[(4-chlorophenyl)sulfonyl](2,5-difluorophenyl) amino]ethyl-5-fluorobenzenebutanoic acid (BMS-299897) alleviates Aβ1–42 seeding and short-term memory deficits in the Aβ25–35 mouse model of Alzheimer's disease;Geniposide attenuates insulin-deficiency-induced acceleration of β-amyloidosis in an APP/PS2 transgenic model of Alzheimer's disease;芝麻素对高糖条件下PC12 细胞Aß 和BACE 1 表达的影响;Geniposide attenuates the level of Aβ1–42 via enhancing leptin signaling in cellular and APP/PS1 transgenic mice;Effects of dietary glucocerebrosides from sea cucumber on the brain sphingolipid profiles of mouse models of Alzheimer's disease.;Cerebrosides from Sea Cucumber Protect Against Oxidative Stress in SAMP8 Mice and PC12 Cells;Mechanisms of DHA-enriched phospholipids in improving cognitive deficits in aged SAMP8 mice with high-fat diet;Punicalagin ameliorates the elevation of plasma homocysteine, amyloid-β, TNF-α and apoptosis by advocating antioxidants and modulating apoptotic mediator proteins in brain modulating apoptotic mediator …;Effects of Astaxanthin and Docosahexaenoic-Acid-Acylated Astaxanthin on Alzheimer's Disease in APP/PS1 Double-Transgenic Mice;Ellagic acid ameliorates learning and memory impairment in APP/PS1 transgenic mice via inhibition of β‑amyloid production and tau hyperphosphorylation;非编码RNA miR-137 调控tau 蛋白磷酸化和L 型钙离子通道CACNA1C 参与阿尔茨海默病的发生;Imbalance of Microglial TLR4/TREM2 in LPS-Treated APP/PS1 Transgenic Mice: A Potential Link Between Alzheimer's Disease and Systemic Inflammation;Repetitive transcranial magnetic stimulation protects mice against 6-OHDA-induced Parkinson's disease symptoms by regulating brain amyloid β level;