ELISA Kit for Amyloid Beta Peptide 1-42 (Ab1-42)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse), Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than

Sample type

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Amyloid Beta Peptide 1-42 (Ab1-42) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Amyloid Beta Peptide 1-42 (Ab1-42) and unlabeled Amyloid Beta Peptide 1-42 (Ab1-42) (Standards or samples) with the pre-coated antibody specific to Amyloid Beta Peptide 1-42 (Ab1-42). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Amyloid Beta Peptide 1-42 (Ab1-42) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Amyloid Beta Peptide 1-42 (Ab1-42) in the sample.

Alternative Names

Item Name

Amyloid Beta Peptide 1-42

Research Area

Neuro science;

Reference