ELISA Kit for Ampicillin (ABPC)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

617.3-50,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 215.7pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ampicillin (ABPC) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ampicillin (ABPC) and unlabeled Ampicillin (ABPC) (Standards or samples) with the pre-coated antibody specific to Ampicillin (ABPC). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ampicillin (ABPC) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Ampicillin (ABPC) in the sample.

Alternative Names

Principen

Item Name

Ampicillin

Research Area

Reference