ELISA Kit for Alpha-1-Antitrypsin (a1AT)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

781.25-200,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Canis familiaris; Canine (Dog)

Sensitivity

The minimum detectable dose of this kit is typically less than 353.94pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Alpha-1-Antitrypsin (a1AT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Alpha-1-Antitrypsin (a1AT) and unlabeled Alpha-1-Antitrypsin (a1AT) (Standards or samples) with the pre-coated antibody specific to Alpha-1-Antitrypsin (a1AT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Alpha-1-Antitrypsin (a1AT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Alpha-1-Antitrypsin (a1AT) in the sample.

Alternative Names

SERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1

Item Name

Alpha-1-Antitrypsin

Research Area

Enzyme & Kinase;Infection immunity;Hepatology;

Reference