ELISA Kit for Adrenomedullin (ADM)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Equus caballus; Equine (Horse)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.92pg/mL

Sample type

serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adrenomedullin (ADM) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adrenomedullin (ADM) and unlabeled Adrenomedullin (ADM) (Standards or samples) with the pre-coated antibody specific to Adrenomedullin (ADM). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adrenomedullin (ADM) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adrenomedullin (ADM) in the sample.

Alternative Names

AM; PAMP; Proadrenomedullin N-20 terminal peptide; ProAM N-terminal 20 peptide

Item Name

Adrenomedullin

Research Area

Endocrinology;Hormone metabolism;

Reference