ELISA Kit for Activin AB (ACVAB)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.62pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Activin AB (ACVAB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Activin AB (ACVAB) and unlabeled Activin AB (ACVAB) (Standards or samples) with the pre-coated antibody specific to Activin AB (ACVAB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Activin AB (ACVAB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Activin AB (ACVAB) in the sample.

Alternative Names

ACV-AB; Activin Beta A Beta B Homodimer

Item Name

Activin AB

Research Area

Endocrinology;Reproductive science;Hormone metabolism;

Reference

Activins and their related proteins in colon carcinogenesis: insights from early and advanced azoxymethane rat models of colon cancer.;