ELISA Kit for A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

31.2-2000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Oryctolagus cuniculus (Rabbit)

Sensitivity

The minimum detectable dose of this kit is typically less than 12.1pg/mL

Sample type

tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

ADMP-1; Aggrecanase-1

Item Name

A Disintegrin And Metalloproteinase With Thrombospondin 4

Research Area

Enzyme & Kinase;Tumor immunity;Infection immunity;Neuro science;Rheumatology;

Reference

Diagnostic utility of fluorogenic peptide-conjugated Au nanoparticle probe corroborated by rabbit model of mild cartilage injury and panel of osteoarthritic …;