ELISA Kit for 5'-Nucleotidase, Cytosolic II (NT5C2)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.312-20ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.128ng/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5'-Nucleotidase, Cytosolic II (NT5C2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 5'-Nucleotidase, Cytosolic II (NT5C2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5'-Nucleotidase, Cytosolic II (NT5C2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5'-Nucleotidase, Cytosolic II (NT5C2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

NT5B; PNT5; GMP; cN-II; NT5CP; Purine 5' Nucleotidase; 5'-Nucleotidase(Purine),Cytosolic Type B; Cytosolic purine 5'-nucleotidase

Item Name

5'-Nucleotidase, Cytosolic II

Research Area

Enzyme & Kinase;

Reference