ELISA Kit for 5',3'-Nucleotidase, Cytosolic (NT5C)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.059ng/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5',3'-Nucleotidase, Cytosolic (NT5C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 5',3'-Nucleotidase, Cytosolic (NT5C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5',3'-Nucleotidase, Cytosolic (NT5C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5',3'-Nucleotidase, Cytosolic (NT5C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

DNT; DNT1; P5N2; PN-I; PN-II; UMPH2; cdN; 5'Nucleotidase,Deoxy(Pyrimidine)Cytosolic Type C; Uridine 5-Prime Monophosphate Hydrolase 2; 5'(3')-deoxyribonucleotidase, cytosolic

Item Name

5',3'-Nucleotidase, Cytosolic

Research Area

Enzyme & Kinase;

Reference