ELISA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

3.12-200ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 1.16ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 4-Hydroxyphenylpyruvate Dioxygenase (HPD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 4-Hydroxyphenylpyruvate Dioxygenase (HPD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 4-Hydroxyphenylpyruvate Dioxygenase (HPD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-Hydroxyphenylpyruvate Dioxygenase (HPD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

PPD; 4-HPPD; 4HPPD; GLOD3; Glyoxalase Domain Containing 3; 4-hydroxyphenylpyruvic acid oxidase

Item Name

4-Hydroxyphenylpyruvate Dioxygenase

Research Area

Enzyme & Kinase;

Reference

Age associated decline in the conversion of leucine to β-Hydroxy-β-Methylbutyrate in rats;