ELISA Kit for 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.312-20ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.117ng/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

SDR12C2; EDH17B3; Short Chain Dehydrogenase/Reductase Family 12C,Member 2; Testosterone 17-Beta-Dehydrogenase 3; Testicular 17-beta-hydroxysteroid dehydrogenase

Item Name

17-Beta-Hydroxysteroid Dehydrogenase Type 3

Research Area

Enzyme & Kinase;

Reference