ELISA Kit for 1,25-Dihydroxyvitamin D3 (DHVD3)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

24.69-2,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 9.92pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to DHVD3 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled DHVD3 analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of DHVD3 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of DHVD3 in the sample.

Alternative Names

DH-VD3; Calcitriol; Rocaltrol; Calcijex; 1,25-(OH)2D3; 1,25-Dihydroxycholecalciferol; 1,25-Dihydroxy Vitamin D3

Item Name

1,25-Dihydroxyvitamin D3

Research Area

Metabolic pathway;Bone metabolism;

Reference

Selective Cytotoxic Action and DNA Damage by Calcitriol-Cu(II) Interaction: Putative Mechanism of Cancer Prevention;Calcium-deficiency assessment and biomarker identification by an integrated urinary metabonomics analysis;Treatment of established left ventricular hypertrophy with fibroblast growth factor receptor blockade in an animal model of CKD;Vitamin D Prevents Podocyte Injury via Regulation of Macrophage M1/M2 Phenotype in Diabetic Nephropathy Rats;Calcitriol?induced?redox?imbalance and DNA breakage in cells sharing a common metabolic feature of malignancies: Interaction with cellular copper (II) ions leads to the production of reactive oxygen species.;Impact of Curcumin and Flaxseed Oil on Bone Turnover in Glucocorticoid-Injected Female Rats;Protective Effect of Soybean and Mushroom against Glucocorticoid-Induced Osteoporosis in Female Rats;Calcitriol–copper interaction leads to non enzymatic, reactive oxygen species mediated DNA breakage and modulation of cellular redox scavengers in hepatocellular …;Effect of Calcitriol on FGF23 Level in Healthy Adults and its Dependence on Phosphate Level;Glucocorticoid exposure induces preeclampsia via dampening 1,25-dihydroxyvitamin D3;Combined effect of pulsed electromagnetic fields and narrowband ultraviolet B on bone metabolism in glucocorticoid-treated rats;Dobesity: relationship between vitamin d deficiency, obesity and sclerostin as a novel biomarker of bone metabolism;