EGLN3 Antibody

Size

100ul

Clone Number

Aliases

Egl 9 family hypoxia inducible factor 3 antibody; Egl nine homolog 3 (C. elegans) antibody; Egl nine homolog 3 antibody; Egl nine like protein 3 isoform antibody; EGL9 homolog of C. elegans 3 antibody; EGLN3 antibody; EGLN3_HUMAN antibody; Factor responsive smooth muscle protein antibody; HIF Prolyl Hydroxylase 3 antibody; HIF-PH3 antibody; HIF-prolyl hydroxylase 3 antibody; HIFP4H3 antibody; HIFPH3 antibody; HPH-1 antibody; HPH-3 antibody; Hypoxia-inducible factor prolyl hydroxylase 3 antibody; P4H3 antibody; PHD3 antibody; Prolyl Hydroxylase Domain Containing Protein 3 antibody; Prolyl hydroxylase domain-containing protein 3 antibody; SM20 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q9H6Z9

Immunogen

Recombinant Human Egl nine homolog 3 protein (1-120AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, IHC; Recommended dilution: IHC:1:20-1:200

Background

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation. Plays a crucial role in DNA damage response (DDR) by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway. Target proteins are preferencially recognized via a LXXLAP motif.

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Conjugate

Non-conjugated

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

EGLN3

Research Areas

Epigenetics and Nuclear Signaling; Cancer; Cardiovascular; Metabolism