EGLN1 Antibody

Size

100ul

Clone Number

Aliases

C1ORF12 antibody; Chromosome 1 Open Reading Frame 12 antibody; DKFZp761F179 antibody; ECYT 3 antibody; ECYT3 antibody; Egl 9 family hypoxia inducible factor 1 antibody; EGL 9 homolog of C. elegans 1 antibody; EGL nine (C.elegans) homolog 1 antibody; Egl nine homolog 1 (C. elegans) antibody; Egl nine homolog 1 antibody; Egl nine like protein 1 antibody; EGLN 1 antibody; Egln1 antibody; EGLN1_HUMAN antibody; HIF PH2 antibody; HIF Prolyl Hydroxylase 2 antibody; HIF-PH2 antibody; HIF-prolyl hydroxylase 2 antibody; HIFP4H 2 antibody; HIFPH2 antibody; HPH 2 antibody; HPH-2 antibody; HPH2 antibody; Hypoxia inducible factor prolyl hydroxylase 2 antibody; Hypoxia-inducible factor prolyl hydroxylase 2 antibody; ORF13 antibody; P4H2 antibody; PHD 2 antibody; PhD2 antibody; PNAS 118 antibody; PNAS 137 antibody; Prolyl Hydroxylase Domain Containing Protein 2 antibody; Prolyl hydroxylase domain-containing protein 2 antibody; Rat Homolog of SM20 antibody; SM 20 antibody; SM-20 antibody; SM20 antibody; Zinc finger MYND domain containing protein 6 antibody; ZMYND6 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q9GZT9

Immunogen

Recombinant Human Egl nine homolog 1 protein (301-426AA)

Raised in

Rabbit

Species Reactivity

Human, Rat

Tested Applications

ELISA, WB, IHC; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200

Background

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferencially recognized via a LXXLAP motif.

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Conjugate

Non-conjugated

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

EGLN1

Research Areas

Epigenetics and Nuclear Signaling; Cancer; Cardiovascular; Metabolism