EGFP/EYFP Monoclonal Antibody

Size

Clone Number

Aliases

GFP; Green Fluorescent Protein; enhanced Green Fluorescent Protein;

Immunogen Species

Bacillus cereus

UniProt ID

C8CHS1

Immunogen

Purified recombinant EYFP.tag full length expressed in E.coli.

Raised in

Mouse

Species Reactivity

Bacillus cereus

Tested Applications

ELISA,WB;Recommended dilution:WB:1:500-1:5000

Background

The green fluorescent protein (GFP) is a protein isolated from the jellyfish Aequorea victoria composed of 238 amino acid residues that exhibits bright green fluorescence. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations. Due to the potential for widespread usage and the evolving needs of researchers, many different mutants of GFP have been engineered. For example, EGFP contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr which result in  brighter green fluorescence. EYFP contains the four amino acid substitutions of Ser-65 to Gly; Val-68 to Leu; Ser-72 to Ala; and Thr-203 to Tyr. EYFP emits yellow fluorescence excited by green light.

Clonality

Monoclonal

Isotype

IgG1

Purification Method

Affinity purified

Conjugate

Non-conjugated

Buffer

Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Form

liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

EGFP/EYFP

Research Areas

Others