DTL Antibody, HRP conjugated

Size

100ug

Clone Number

Aliases

Lethal(2) denticleless protein homolog antibody; CDW1 antibody; DCAF2 antibody; DDB1 and CUL4 associated factor 2 antibody; Ddb1- and Cul4-associated factor 2 antibody; Denticleless homolog antibody; Denticleless homolog (Drosophila) antibody; Denticleless protein homolog antibody; Dtl antibody; DTL_HUMAN antibody; L2DTL antibody; Lethal(2) denticleless protein homolog antibody; RA regulated nuclear matrix associated protein antibody; RAMP antibody; Retinoic acid regulated nuclear matrix associated protein antibody; Retinoic acid-regulated nuclear matrix-associated protein antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q9NZJ0

Immunogen

Recombinant Human Denticleless protein homolog protein (393-550AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA

Background

Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1), FBXO18/FBH1 and KMT5A (PubMed:16861906, PubMed:16949367, PubMed:16964240, PubMed:17085480, PubMed:18703516, PubMed:18794347, PubMed:18794348, PubMed:19332548, PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication (PubMed:16861906, PubMed:16949367, PubMed:17085480). CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing (PubMed:18794348, PubMed:19332548). KMT5A degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration (PubMed:23478445). Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the \'K+4\' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the \'Lys-164\' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis (PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613).

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

HRP

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

DTL

Research Areas

Cell biology