Cusabio
SKU:CSB-RA964680A0HU
GNA13 Recombinant Monoclonal Antibody
GNA13 Recombinant Monoclonal Antibody
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| Release Date | 2026-10-27 |
|---|---|
| Product Code | CSB-RA964680A0HU |
| Short Name | GNA13 Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IF, FC |
| Dilution | WB:1:500-1:5000, IF:1:20-1:200, FC:1:20-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium Azide, pH 7.4. |
| Antigen | A synthesized peptide derived from human GNA13 |
| Antigen Species | Human |
| Uniprot ID | Q14344 |
| Target Name | GNA13 |
| Clonality | Monoclonal |
| Clone ID | 5A11 |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12951948/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: MCF7 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), U2OS whole cell lysate(30µg), A549 whole cell lysate(30µg), Raji whole cell lysate(30µg）, HEK293 whole cell lysate(30µg）, HepG2 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg)
All lanes: GNA13 antibody at 1:1000 incubated at room temperature for 1h
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time：2min
Image 2
Immunofluorescence staining of PC-3 cell with CSB-RA964680A0HU at 1:50 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 3
Overlay Peak curve showing PC-3 cells stained with CSB-RA964680A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10<sup>6</sup>cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.
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