PCBP2 Recombinant Monoclonal Antibody

Image 1

Western Blot

Positive WB detected in:MCF7 whole cell lysate(30µg), K562 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), A549 whole cell lysate(30µg), Hela whole cell lysate(30µg), Jurkat whole cell lysate(30µg), COLO205 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg)

All lanes: PCBP2 antibody at 1:1000

Secondary

Goat polyclonal to rabbit IgG at 1/40000 dilution

Predicted band size: 35, 39 kDa

Observed band size: 39 kDa

Exposure time：1min

Image 2

IHC image of CSB-RA955026A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Image 3

Immunofluorescence staining of hela cell with CSB-RA955026A0HU at 1:50 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Image 4

Overlay Peak curve showing Raji cells stained with CSB-RA955026A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.