Cusabio
SKU:CSB-RA942071A0HU
NFE2 Recombinant Monoclonal Antibody
NFE2 Recombinant Monoclonal Antibody
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| Release Date | 2025-07-11 |
|---|---|
| Product Code | CSB-RA942071A0HU |
| Short Name | NFE2 Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IF |
| Dilution | WB:1:500-1:2000, IF:1:50-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium Azide, pH 7.4. |
| Antigen | A synthesized peptide derived from human NFE2 |
| Antigen Species | Human |
| Uniprot ID | Q16621 |
| Target Name | NFE2 |
| Clonality | Monoclonal |
| Clone ID | 1H11 |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12943574/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: Jurkat whole cell lysate(30µg) , U-937 whole cell lysate(30µg) , HL-60 whole cell lysate(30µg) , Hela whole cell lysate(30µg) , NIH/3T3 whole cell lysate(30µg)
All lanes: NFE2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 41 kDa
Observed band size: 50 kDa
Exposure time：3min
Image 2
Immunofluorescence staining of HepG2 cell with CSB-RA942071A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .
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