Cusabio
SKU:CSB-RA922204A0HU
NDUFS2 Recombinant Monoclonal Antibody
NDUFS2 Recombinant Monoclonal Antibody
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| Release Date | 2026-04-30 |
|---|---|
| Product Code | CSB-RA922204A0HU |
| Short Name | NDUFS2 Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IHC, FC |
| Dilution | WB:1:500-1:5000, IHC:1:50-1:200, FC:1:20-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium Azide, pH 7.4. |
| Antigen | A synthesized peptide derived from human NDUFS2 |
| Antigen Species | Human |
| Uniprot ID | O75306 |
| Target Name | NDUFS2 |
| Clonality | Monoclonal |
| Clone ID | 3C1 |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12950328/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: THP-1 whole cell lysate(30µg), SY5Y whole cell lysate(30µg), A549 whole cell lysate(30µg), Hela whole cell lysate(30µg), HEK293 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg)
All lanes: NDUFS2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 53kDa
Observed band size: 53 kDa
Exposure time：30s
Image 2
IHC image of CSB-RA922204A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Image 3
IHC image of CSB-RA922204A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Image 4
Overlay Peak curve showing HepG2 cells stained with CSB-RA922204A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10<sup>6</sup>cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.
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