Cusabio
SKU:CSB-RA915422A0HU
STAT5A/STAT5B Recombinant Monoclonal Antibody
STAT5A/STAT5B Recombinant Monoclonal Antibody
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| Release Date | 2025-07-11 |
|---|---|
| Product Code | CSB-RA915422A0HU |
| Short Name | STAT5A/STAT5B Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC |
| Dilution | WB:1:500-1:2000, IHC:1:50-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium Azide, pH 7.4. |
| Antigen | A synthesized peptide derived from human STAT5A/STAT5B |
| Antigen Species | Human |
| Uniprot ID | P42229/P51692 |
| Target Name | STAT5A/STAT5B |
| Clonality | Monoclonal |
| Clone ID | 3H9 |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12942749/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: THP-1 whole cell lysate(20µg) , A549 whole cell lysate(20µg) ,K-562 whole cell lysate(20µg) ,Hela whole cell lysate(20µg) ,NIH/3T3 whole cell lysate(20µg) ,JURKAT whole cell lysate(20µg) , Mouse liver tissue lysate(20µg)
All lanes: STAT5A/B antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 90 kDa
Observed band size: 102 kDa
Exposure time：40s
Image 2
IHC image of CSB-RA915422A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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