Icam2 Recombinant Monoclonal Antibody

Image 1

Western Blot

Positive WB detected in: HEK293 whole cell lysate(30µg), HEK293T whole cell lysate(30µg), Mouse lung tissue lysate(30µg)

All lanes: ICAM 2 antibody at 1:1000

Secondary

Goat polyclonal to rabbit IgG at 1/40000 dilution

Predicted band size: 31 kDa

Observed band size: 60 kDa

Exposure time：3min

Image 2

IHC image of CSB-RA759127A0MO diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Image 3

IHC image of CSB-RA759127A0MO diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Image 4

Overlay Peak curve showing Raji cells stained with CSB-RA759127A0MO (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.