Cusabio
SKU:CSB-RA246132A0HU
MRPL28 Recombinant Monoclonal Antibody
MRPL28 Recombinant Monoclonal Antibody
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| Release Date | 2025-10-28 |
|---|---|
| Product Code | CSB-RA246132A0HU |
| Short Name | MRPL28 Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, FC |
| Dilution | WB:1:500-1:5000, FC:1:50-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium Azide, pH 7.4. |
| Antigen | A synthesized peptide from human MRPL28 protein |
| Antigen Species | Human |
| Uniprot ID | Q13084 |
| Target Name | MRPL28 |
| Clonality | Monoclonal |
| Clone ID | 14B4 |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12946065/ |
| Size | 100ul |
Image 1
Western Blot
,
Positive WB detected in:HepG2 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), HEK293T whole cell lysate(30µg), Hela whole cell lysate(30µg), Jurkat whole cell lysate(30µg), MCF7 whole cell lysate(30µg), Mouse kidney tissue lysate(30µg), Rat kidney tissue lysate(30µg)
All lanes: CtBP2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time：10s
Image 2
Overlay Peak curve showing Hela cells stained with CSB-RA246132A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10<sup>6</sup>cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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