Cusabio
SKU:CSB-RA185642A0HU
POLB Recombinant Monoclonal Antibody
POLB Recombinant Monoclonal Antibody
Couldn't load pickup availability
| Release Date | 2025-07-11 |
|---|---|
| Product Code | CSB-RA185642A0HU |
| Short Name | POLB Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IHC |
| Dilution | WB:1:500-1:2000, IHC:1:50-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium Azide, pH 7.4. |
| Antigen | A synthesized peptide derived from human POLB |
| Antigen Species | Human |
| Uniprot ID | P06746 |
| Target Name | POLB |
| Clonality | Monoclonal |
| Clone ID | 6F4 |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12943579/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: K562 whole cell lysate(30µg) , PC-3 whole cell lysate(30µg) , Hela whole cell lysate(30µg) , Jurkat whole cell lysate(30µg) , Mouse brain tissue lysate(30µg) , Rat brain tissue lysate(30µg)
All lanes: DNA polymerase beta antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time：3min
Image 2
IHC image of CSB-RA185642A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Share
