Cusabio
SKU:CSB-RA176457A0HU
TUBA4A/TUBA1B Recombinant Monoclonal Antibody
TUBA4A/TUBA1B Recombinant Monoclonal Antibody
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| Release Date | 2025-07-11 |
|---|---|
| Product Code | CSB-RA176457A0HU |
| Short Name | TUBA4A/TUBA1B Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC |
| Dilution | WB:1:500-1:2000, IHC:1:50-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium Azide, pH 7.4. |
| Antigen | A synthesized peptide derived from human TUBA4A/TUBA1B |
| Antigen Species | Human |
| Uniprot ID | P68363/P68366 |
| Target Name | TUBA4A/TUBA1B |
| Clonality | Monoclonal |
| Clone ID | 1F7 |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12942747/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: HEK293 whole cell lysate(30µg) , JURKAT whole cell lysate(30µg) ,U-251MG whole cell lysate(30µg) ,A549 whole cell lysate(30µg)
All lanes: alpha 1B/4A Tubulin antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 50 kDa
Observed band size: 55 kDa
Exposure time：1min
Image 2
IHC image of CSB-RA176457A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Image 3
IHC image of CSB-RA176457A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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