Cusabio
SKU:CSB-RA167825A0HU
MAPK8/MAPK10 Recombinant Monoclonal Antibody
MAPK8/MAPK10 Recombinant Monoclonal Antibody
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| Release Date | 2026-10-27 |
|---|---|
| Product Code | CSB-RA167825A0HU |
| Short Name | MAPK8/MAPK10 Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IF, FC |
| Dilution | WB:1:500-1:5000, IF:1:20-1:200, FC:1:20-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium Azide, pH 7.4. |
| Antigen | A synthesized peptide derived from human MAPK8/MAPK10 |
| Antigen Species | Human |
| Uniprot ID | P45983/P53779 |
| Target Name | MAPK8/MAPK10 |
| Clonality | Monoclonal |
| Clone ID | 34G8 |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12951808/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: HEK293T whole cell lysate(30µg)
All lanes: MAPK8/MAPK10 antibody at 1:1000 incubated at room temperature for 1h
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 48, 53 kDa
Observed band size: 48, 53 kDa
Exposure time：2min
Image 2
Immunofluorescence staining of HeLa cell with CSB-RA167825A0HU at 1:50 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 3
Overlay Peak curve showing HeLa cells stained with CSB-RA167825A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10<sup>6</sup>cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.
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