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Cusabio

SKU:CSB-RA152716A0HU

BCL2A1 Recombinant Monoclonal Antibody

BCL2A1 Recombinant Monoclonal Antibody

Regular price $210.00 USD
Regular price Sale price $210.00 USD
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Release Date 2025-07-11
Product Code CSB-RA152716A0HU
Short Name BCL2A1 Recombinant Monoclonal Antibody
Antibody Type Recombinant Antibody
List Price $350/100ul, $210/50ul
Species Reactivity Human
Tested Applications ELISA, IF, FC
Dilution IF:1:50-1:200, FC:1:50-1:200
Purification Method Affinity chromatography
Buffer Formulation PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium Azide, pH 7.4.
Antigen A synthesized peptide derived from human BCL2A1
Antigen Species Human
Uniprot ID Q16548
Target Name BCL2A1
Clonality Monoclonal
Clone ID 11G11
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/12943731/
Size 50ul

Image 1

Immunofluorescence staining of A549 cell with CSB-RA152716A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .

Image 2

Overlay Peak curve showing Raji cells stained with CSB-RA152716A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10<sup>6</sup>cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.

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