Cusabio
SKU:CSB-RA149810A0HU
EEF1G Recombinant Monoclonal Antibody
EEF1G Recombinant Monoclonal Antibody
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| Release Date | 2025-07-11 |
|---|---|
| Product Code | CSB-RA149810A0HU |
| Short Name | EEF1G Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IF, FC |
| Dilution | WB:1:500-1:2000, IF:1:50-1:200, FC:1:50-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium Azide, pH 7.4. |
| Antigen | A synthesized peptide derived from human EEF1G |
| Antigen Species | Human |
| Uniprot ID | P26641 |
| Target Name | EEF1G |
| Clonality | Monoclonal |
| Clone ID | 6F1 |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12942855/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate(20µg) ,Hela whole cell lysate(20µg) , A549 whole cell lysate(20µg) , COLO-205 whole cell lysate(20µg) ,HT-29 whole cell lysate(20µg) ,PC-3 whole cell lysate(20µg)
All lanes: eEF1B gamma antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 50 kDa
Observed band size: 55 kDa
Exposure time：20s
Image 2
Immunofluorescence staining of Hela cell with CSB-RA149810A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .
Image 3
Overlay Peak curve showing BxPC-3 cells stained with CSB-RA149810A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10<sup>6</sup>cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.
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