Cusabio
SKU:CSB-RA081385A0HU
CUL4A Recombinant Monoclonal Antibody
CUL4A Recombinant Monoclonal Antibody
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| Release Date | 2025-07-11 |
|---|---|
| Product Code | CSB-RA081385A0HU |
| Short Name | CUL4A Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC, IF |
| Dilution | WB:1:500-1:2000, IHC:1:50-1:200, IF:1:50-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium Azide, pH 7.4. |
| Antigen | A synthesized peptide derived from human CUL4A |
| Antigen Species | Human |
| Uniprot ID | Q13619 |
| Target Name | CUL4A |
| Clonality | Monoclonal |
| Clone ID | 5A10 |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12942891/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: HL-60 whole cell lysate(30µg) ,U-937 whole cell lysate(30µg) , HepG2 whole cell lysate(30µg) ,COLO-205 whole cell lysate(30µg)
All lanes: Cullin 4A antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 88 kDa
Observed band size: 88 kDa
Exposure time：2min
Image 2
IHC image of CSB-RA081385A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Image 3
Immunofluorescence staining of Hela cell with CSB-RA081385A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .
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