Cusabio
SKU:CSB-RA024405MA5HU
TREM2 Recombinant Monoclonal Antibody
TREM2 Recombinant Monoclonal Antibody
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| Release Date | 2025-10-16 |
|---|---|
| Product Code | CSB-RA024405MA5HU |
| Short Name | TREM2 Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IF |
| Dilution | WB:1:500-1:2000, IF:1:50-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant Human TREM2 protein |
| Antigen Species | Human |
| Uniprot ID | Q9NZC2 |
| Target Name | TREM2 |
| Clonality | Monoclonal |
| Clone ID | 22E5 |
| Host Species | Human |
| Isotype | IgG1 |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12945857/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: A2780 whole cell lysate(30µg),U87 whole cell lysate(30µg)
All lanes: TREM2 antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 25.447,24.328,24,669 kDa
Observed band size: 50 kDa
Exposure time: 120s
Image 2
Immunofluorescence staining of NIH/3T3 cell with CSB-RA024405MA5HU at 1:30 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.
Image 3
The Binding Activity of Mouse Trem2 with Anti-TREM2 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Trem2 (CSB-MP024405MOd7) at 2 μg/mL can bind Anti-TREM2 recombinant antibody. The EC50 is 1.726-2.010 ng/mL.
Image 4
The Binding Activity of Mouse Trem2 with Anti-TREM2 Recombinant Antibody
Activity: TREM2 Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Mouse Trem2 (CSB-MP024405MOd7) with an affinity constant of 0.139 nM as detected by MetaSPR Assay (WeSPRTM 200).
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