Cusabio
SKU:CSB-RA021189MA1HU
SGK1 Recombinant Monoclonal Antibody
SGK1 Recombinant Monoclonal Antibody
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| Release Date | 2025-07-11 |
|---|---|
| Product Code | CSB-RA021189MA1HU |
| Short Name | SGK1 Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, IF, FC |
| Dilution | IF:1:50-1:200, FC:1:50-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant Human SGK1 protein |
| Antigen Species | Human |
| Uniprot ID | O00141 |
| Target Name | SGK1 |
| Clonality | Monoclonal |
| Clone ID | 2F3 |
| Host Species | Human |
| Isotype | IgG1 |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12943528/ |
| Size | 50ul |
Image 1
Immunofluorescence staining of Hela cell with CSB-RA021189MA1HU at 1:30 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG(H+L) .
Image 2
Immunofluorescence staining of HepG2 cell with CSB-RA021189MA1HU at 1:30 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG(H+L) .
Image 3
Overlay Peak curve showing Hela cells stained with CSB-RA021189MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10<sup>6</sup>cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35 min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.
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