Cusabio
SKU:CSB-RA017494MA1HU
PAX8 Recombinant Monoclonal Antibody
PAX8 Recombinant Monoclonal Antibody
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| Release Date | 2025-10-16 |
|---|---|
| Product Code | CSB-RA017494MA1HU |
| Short Name | PAX8 Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, FC, SPR |
| Dilution | ELISA:1:5000-1:50000, FC:1:50-1:500 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant Human PAX8 protein |
| Antigen Species | Human |
| Uniprot ID | Q06710 |
| Target Name | PAX8 |
| Clonality | Monoclonal |
| Clone ID | 16C5 |
| Host Species | Human |
| Isotype | IgG1 |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12945770/ |
| Size | 50ul |
Image 1
The Binding Activity of Human PAX8 with Anti-PAX8 Recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human PAX8 (CSB-MP017494HU2) at 2 μg/mL can bind Anti-PAX8 recombinant antibody. The EC<sub>50</sub> is 0.6325-0.7353 ng/mL.
Image 2
Overlay Peak curve showing 786-O cells stained with CSB-RA017494MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10<sup>6</sup>cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.
Image 3
The Binding Activity of Human PAX8 with Anti-PAX8 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human PAX8 (CSB-EP017494HU2) at 2 μg/mL can bind Anti-PAX8 recombinant antibody. The EC50 is 0.8357-0.9576 ng/mL.
Image 4
The Binding Activity of Human PAX8 with Anti-PAX8 Recombinant Antibody
Activity: PAX8 Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Human PAX8 (CSB-EP017494HU2) with an affinity constant of 0.346 nM as detected by MetaSPR Assay (WeSPRTM 200).
Image 5
The Binding Activity of Human PAX8 with Anti-PAX8 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human PAX8 (CSB-EP017494HUc7) at 2 μg/mL can bind Anti-PAX8 recombinant antibody. The EC50 is 1.242-1.392 ng/mL.
Image 6
The Binding Activity of Human PAX8 with Anti-PAX8 Recombinant Antibody
Activity: PAX8 Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Human PAX8 (CSB-EP017494HUc7) with an affinity constant of 0.296 nM as detected by MetaSPR Assay (WeSPRTM 200).
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