Cusabio
SKU:CSB-RA012704MA1HU
L1CAM Recombinant Monoclonal Antibody
L1CAM Recombinant Monoclonal Antibody
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| Release Date | 2025-10-16 |
|---|---|
| Product Code | CSB-RA012704MA1HU |
| Short Name | L1CAM Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human, Mouse, Macaca mulatta |
| Tested Applications | ELISA, WB, IHC, FC |
| Dilution | ELISA:1:5000-1:50000, WB:1:500-1:2000, IHC:1:50-1:200, FC:1:50-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant Human L1CAM protein |
| Antigen Species | Human |
| Uniprot ID | P32004 |
| Target Name | L1CAM |
| Clonality | Monoclonal |
| Clone ID | Ab2c2 |
| Host Species | Human |
| Isotype | IgG1 |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12945756/ |
| Size | 50ul |
Image 1
The Binding Activity of Macaca fascicularis L1CAM with Anti-L1CAM recombinant antibody
Activity: Measured by its binding ability in a functional ELISA.Immobilized Macaca fascicularis L1CAM(CSB-MP6775MOV) at 2 μg/mL can bind Anti-L1CAM recombinant antibody. The EC<sub>50</sub> is 1.496-1.709 ng/mL.
Image 2
The Binding Activity of Human L1CAM with Anti-L1CAM recombinant antibody
Activity: Measured by its binding ability in a functional ELISA.Immobilized Human L1CAM(CSB-MP012704HU1) at 2 μg/mL can bind Anti-L1CAM recombinant antibody. The EC<sub>50</sub> is 5.356-6.664 ng/mL.
Image 3
The Binding Activity of Mouse L1cam with Anti-L1CAM recombinant antibody
Activity: Measured by its binding ability in a functional ELISA.Immobilized Mouse L1cam(CSB-MP012704MO) at 2 μg/mL can bind Anti-L1CAM recombinant antibody. The EC<sub>50</sub> is 1.827-2.141 ng/mL.
Image 4
Western Blot
Positive WB detected in: HT29 whole cell lysate(30µg)
All lanes: L1CAM antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 140 kDa
Observed band size: 200 kDa
Exposure time: 2min
Image 5
IHC image of CSB-RA012704MA1HU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.
Image 6
Overlay Peak curve showing Hela cells stained with CSB-RA012704MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10<sup>6</sup>cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35 min at 4℃.Control antibody (green line) was human IgG1a (1ug/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.
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