Cusabio
SKU:CSB-RA008727MA1HU
FLOT1 Recombinant Monoclonal Antibody
FLOT1 Recombinant Monoclonal Antibody
Couldn't load pickup availability
| Release Date | 2025-07-11 |
|---|---|
| Product Code | CSB-RA008727MA1HU |
| Short Name | FLOT1 Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IF, FC |
| Dilution | WB:1:500-1:2000, IF:1:50-1:200, FC:1:50-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant Human FLOT1 protein |
| Antigen Species | Human |
| Uniprot ID | O75955 |
| Target Name | FLOT1 |
| Clonality | Monoclonal |
| Clone ID | 4C8B6 |
| Host Species | Human |
| Isotype | IgG1 |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12942678/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: HEK293 whole cell lysate(20µg) , U-251MG whole cell lysate(20µg) , HeLa whole cell lysate(20µg) , Raji whole cell lysate(20µg) , A549 whole cell lysate(20µg) , HepG2 whole cell lysate(20µg)
All lanes: FLOT1 antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time：1min
Image 2
Western Blot
Positive WB detected in: Rat brain tissue lysate(20µg)
All lanes: FLOT1 antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 47.4 kDa
Observed band size: 48 kDa
Exposure time: 2min
Image 3
Immunofluorescence staining of NIH/3T3 cell with CSB-RA008727MA1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.
Image 4
Immunofluorescence staining of Hela cell with CSB-RA008727MA1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.
Image 5
Overlay Peak curve showing A431 cells stained with CSB-RA008727MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10<sup>6</sup>cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.
Share
