Cusabio
SKU:CSB-RA007670MA1HU
ENO1 Recombinant Monoclonal Antibody
ENO1 Recombinant Monoclonal Antibody
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| Release Date | 2025-07-11 |
|---|---|
| Product Code | CSB-RA007670MA1HU |
| Short Name | ENO1 Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IHC, IF, FC |
| Dilution | WB:1:500-1:2000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant Human ENO1 protein |
| Antigen Species | Human |
| Uniprot ID | P06733 |
| Target Name | ENO1 |
| Clonality | Monoclonal |
| Clone ID | 8H9G12 |
| Host Species | Human |
| Isotype | IgG1 |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12942679/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: HeLa whole cell lysate(20µg) , U-251MG whole cell lysate(20µg) , A549 whole cell lysate(20µg) , PC-3 whole cell lysate(20µg) , K562 whole cell lysate(20µg) , NIH/3T3 whole cell lysate(20µg) , HEK293 whole cell lysate(20µg) , Jurkat whole cell lysate(20µg) , HepG2 whole cell lysate(20µg)
All lanes: ENO1 antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 47 kDa
Observed band size: 55 kDa
Exposure time：5s
Image 2
Western Blot
Positive WB detected in: Mouse brain tissue lysate(20µg) , Rat brain tissue lysate(20µg) , Mouse kidney tissue lysate(20µg) , Rat kidney tissue lysate(20µg) , Mouse lung tissue lysate(20µg) , Mouse spleen tissue lysate(20µg)
All lanes: ENO1 antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 47.2 kDa
Observed band size: 55 kDa
Exposure time: 2min
Image 3
IHC image of CSB-RA007670MA1HU diluted at 1:50 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Image 4
Immunofluorescence staining of HepG2 cell with CSB-RA007670MA1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.
Image 5
Immunofluorescence staining of MCF-7 cell with CSB-RA007670MA1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.
Image 6
Overlay Peak curve showing Hela cells stained with CSB-RA007670MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10<sup>6</sup>cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.
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