CXCR5 Recombinant Monoclonal Antibody

Image 1

Western Blot

Positive WB detected in: Jurkat whole cell lysate(30µg), Raji whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), U-87MG whole cell lysate(30µg), CT26 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), HL-60 whole cell lysate(30µg)

All lanes: CXCR5 antibody at 1:1000

Secondary

Goat polyclonal to human IgG at 1/40000 dilution

Predicted band size: 42 kDa

Observed band size: 33 kDa

Exposure time: 2min

Image 2

IHC image of CSB-RA006255MA1HU diluted at 1:50 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.

Image 3

Immunofluorescence staining of HepG2 cell with CSB-RA006255MA1HU at 1:30 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.

Image 4

Overlay Peak curve showing Raji cells stained with CSB-RA006255MA1HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.