Cusabio
SKU:CSB-PA889157LA01HU
USE1 Antibody
USE1 Antibody
Couldn't load pickup availability
| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA889157LA01HU |
| Short Name | USE1 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IF, IP |
| Dilution | WB:1:500-1:5000, IF:1:50-1:500, IP:1:200-1:2000 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Vesicle transport protein USE1 protein (1-146aa) |
| Antigen Species | Human |
| Uniprot ID | Q9NZ43 |
| Target Name | USE1 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12550860/ |
| Size | 100ug |
Image 1
Western Blot
Positive WB detected in: Jurkat whole cell lysate, Mouse heart tissue, Mouse brain tissue, Mouse kidney tissue, Mouse stomach tissue, Mouse skeletal muscle tissue
All lanes: USE1 antibody at 2.8ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 30, 17, 16 kDa
Observed band size: 36 kDa
Image 2
Immunofluorescent analysis of U251 cells using CSB-PA889157LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Image 3
Immunoprecipitating USE1 in PC-3 whole cell lysate
Lane 1: Rabbit control IgG (1ug) instead of CSB-PA889157LA01HU in PC-3 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA889157LA01HU (6ug) + PC-3 whole cell lysate (500ug)
Lane 3: PC-3 whole cell lysate (10ug)
Share
